Immunocytochemistry/Immunofluorescence analysis using mouse fibroblast cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with Ubiquitin monoclonal antibody (Product # MA5-45330) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was DAPI (blue) nuclear stain at 1:5,000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.